Characterization of Haemophilus paragallinarum Isolated in the Philippines.

Effects of transformation on the hemagglutinins of Haemophilus paragallinarum.

Strain 0083 and two field isolates of H. paragallinarum were previously converted into NAD-independent organisms by the use of crude DNA extractions from naturally occurring NAD-independent H. paragallinarum isolates. Two of these transformed isolates [0083(T) and A745(T)] were used as DNA donors in another transformation experiment in which another field isolate (M85) was used as the DNA recip...

Monoclonal antibody characterization of South African field isolates of Haemophilus paragallinarum.

A total of 27 different isolates of Haemophilus paragallinarum were made from chickens between June 1991 and December 1992. All of these isolates were examined by ELISA, by means of a locally produced panel of three monoclonal antibodies (denoted F1, V1 and VF3). The isolates were all of the F1 antigenic type. Three of them showed a weak reaction with the F1 monoclonal antibody, while three oth...

Haemophilus paragallinarum haemagglutinin: role in adhesion, serotyping and pathogenicity.

It is suggested that Haemophilus paragallinarum requires at least three haemagglutinins for adhesion during infection. This paper reports the partial purification and characterization of the HA-L haemagglutinin from H. paragallinarum strain 46-C3, a heat sensitive, trypsin sensitive haemagglutinin that has been shown to be the serovar specific haemagglutinin in this organism. Using the pl and m...

Infectious coryza due to Haemophilus paragallinarum serovar B in China.

Serological classification of Haemophilus paragallinarum with a hemagglutinin system.

Antigens, prepared from 17 strains of Haemophilus paragallinarum by treatment with potassium thiocyanate followed by sonication, uniformly agglutinated glutaraldehyde-fixed chicken erythrocytes and formed specific hemagglutination inhibition antibodies in rabbits. Attempts were made to classify the strains into serotypes by a combination of cross-hemagglutination inhibition and cross-absorption...